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Israel warned Iranians this morning that their lives would be at risk if they use the country’s railways on Tuesday before the end of a negotiating deadline imposed by Donald Trump with a threat to destroy Iran’s bridges and power plants.

Israel’s military, writing in Farsi, said in a social media post that “from this moment” – 8.50am Iran time – and “until 21:00 Iran time” Iranians should refrain from “traveling by train throughout Iran” for the sake of their own security.

“Your presence on trains and near railway lines endangers your life,” the statement continued, a clear warning that stations and tracks normally used by civilians could be bombed on Tuesday.

The threat comes hours before an ultimatum set by the US president expires at 8pm ET on Tuesday (1am in London), or 4.30am on Wednesday in Iran, in an attempt by the US to force major concessions from Iran with the threat of escalation.

At a White House press conference on Monday, Trump said that Iran “can be taken out in one night and that night might be tomorrow”, reiterating threats to bomb its power plants and bridges in a concentrated attack.

Iran on Monday rejected a proposal to implement an immediate ceasefire followed by peace negotiations brokered by Pakistan, Egypt and Turkey, arguing that it wanted a permanent end to the war. It issued a 10-point counterproposal, which Trump acknowledged but said was “not good enough”.

The prospect of bombing the country’s infrastructure has been condemned by lawyers and experts as a probable war crime because its impact on civilians would be disproportionate to whatever notional military advantage is gained, a conclusion that has been dismissed by the Trump administration.

Negotiations continued on Tuesday morning, though there were few clear developments. On X, Iran’s president, Masoud Pezeshkian, said: “Over 14 million proud Iranians have, up to this moment, declared their readiness to sacrifice their lives in defense of Iran. I too have been, am, and will be a sacrificer for Iran.”

On Monday, Pete Hegseth, the US secretary of defence, said that “today will be the largest volume of strikes” on Iran and that attacks on Tuesday would be “even more than today” as the negotiating deadline loomed.

Iranian media reported on Tuesday that Khorramabad airport in western Iran had been attacked, while Israel said it had conducted another wave of strikes on Tehran overnight. Israel’s military also said it had bombed a petrochemical facility in Shiraz, where it said nitric acid used to make explosives is produced, as well as a ballistic missile launch site in north-western Iran.

US officials also told Fox News and the Wall Street Journal that B-2 stealth bombers had dropped 30,000lb “bunker buster” bombs on an Islamic Revolutionary Guard Corps compound in Tehran on Saturday. The bombs were GBU-57 munitions, the type used in last June’s attack by the US on Iran’s underground nuclear facility at Fordow.

Israeli media reported that the prime minister, Benjamin Netanyahu, told members of the country’s security cabinet on Sunday that the war against Hezbollah in Lebanon would continue regardless of what happened in the negotiations between the US and Iran. There was, the prime minister said, a “separation of theatres”.

An attack on Saudi Arabia hit a petrochemical complex in a sprawling industrial area in the eastern city of Jubail and workers at the site had to be evacuated.

Sirens were repeatedly sounded in Israel as missile attacks continued. Five impacts were reported in the Tel Aviv area as Israel said that Iran had fired ballistic missiles with cluster warheads, but no casualties were immediately reported.

The price of Brent crude oil increased marginally to just above $110 (£83) a barrel in morning trading.